Review





Similar Products

t241p  (Addgene inc)
91
Addgene inc t241p
Example of raw data taken from Envision plate reader, along with NanoBRET calculations
T241p, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t241p/product/Addgene inc
Average 91 stars, based on 1 article reviews
t241p - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
wt construct  (Addgene inc)
91
Addgene inc wt construct
Example of raw data taken from Envision plate reader, along with NanoBRET calculations
Wt Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wt construct/product/Addgene inc
Average 91 stars, based on 1 article reviews
wt construct - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
phack gal4 donr t2a flp1  (Addgene inc)
91
Addgene inc phack gal4 donr t2a flp1
Example of raw data taken from Envision plate reader, along with NanoBRET calculations
Phack Gal4 Donr T2a Flp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phack gal4 donr t2a flp1/product/Addgene inc
Average 91 stars, based on 1 article reviews
phack gal4 donr t2a flp1 - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
pa 19477  (Rohm and Haas)
90
Rohm and Haas pa 19477
Example of raw data taken from Envision plate reader, along with NanoBRET calculations
Pa 19477, supplied by Rohm and Haas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pa 19477/product/Rohm and Haas
Average 90 stars, based on 1 article reviews
pa 19477 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Example of raw data taken from Envision plate reader, along with NanoBRET calculations

Journal: STAR Protocols

Article Title: Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay

doi: 10.1016/j.xpro.2023.102461

Figure Lengend Snippet: Example of raw data taken from Envision plate reader, along with NanoBRET calculations

Article Snippet: pCMV5-BRAF REG -Halo variants , Spencer-Smith et al. 2022 , Available at Addgene.org WT cat# 194771 T241P cat# 202549.

Techniques: Construct

Examples of BRAF autoinhibition assays with BRET readings normalized to the average of the WT-NanoBRET pair

Journal: STAR Protocols

Article Title: Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay

doi: 10.1016/j.xpro.2023.102461

Figure Lengend Snippet: Examples of BRAF autoinhibition assays with BRET readings normalized to the average of the WT-NanoBRET pair

Article Snippet: pCMV5-BRAF REG -Halo variants , Spencer-Smith et al. 2022 , Available at Addgene.org WT cat# 194771 T241P cat# 202549.

Techniques:

NanoBRET BRAF autoinhibition assay NanoBRET assay measuring the interaction of WT or T241P BRAF REG -Halo and WT Nano-BRAF CAT . Also shown are the effects of the CAT domain V600E mutation and co-expression of HA- tagged KRAS G12V with the WT NanoBRET pair. Data points represent BRET signals (normalized to WT set at 100) of quadruplicate wells from 3 independent experiments ± SD. Student’s t test (two-tailed, assuming unequal variance) was performed. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Cell lysates were examined by immunoblot analysis for BRAF REG , BRAF CAT , and HA-KRAS levels.

Journal: STAR Protocols

Article Title: Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay

doi: 10.1016/j.xpro.2023.102461

Figure Lengend Snippet: NanoBRET BRAF autoinhibition assay NanoBRET assay measuring the interaction of WT or T241P BRAF REG -Halo and WT Nano-BRAF CAT . Also shown are the effects of the CAT domain V600E mutation and co-expression of HA- tagged KRAS G12V with the WT NanoBRET pair. Data points represent BRET signals (normalized to WT set at 100) of quadruplicate wells from 3 independent experiments ± SD. Student’s t test (two-tailed, assuming unequal variance) was performed. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Cell lysates were examined by immunoblot analysis for BRAF REG , BRAF CAT , and HA-KRAS levels.

Article Snippet: pCMV5-BRAF REG -Halo variants , Spencer-Smith et al. 2022 , Available at Addgene.org WT cat# 194771 T241P cat# 202549.

Techniques: Mutagenesis, Expressing, Two Tailed Test, Western Blot

Journal: STAR Protocols

Article Title: Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay

doi: 10.1016/j.xpro.2023.102461

Figure Lengend Snippet:

Article Snippet: pCMV5-BRAF REG -Halo variants , Spencer-Smith et al. 2022 , Available at Addgene.org WT cat# 194771 T241P cat# 202549.

Techniques: Recombinant, Western Blot, Staining, Construct, Software, Cell Counting, Transferring, Sterility